The ability of Pst27791 to enhance Pst colonization was compromised when TaRaf46 was silenced, suggesting that the virulence of Pst27791 is mediated by TaRaf46.
Pst27791 may stabilize TaRaf46 through the inhibition of proteasome-mediated degradation in N. The overexpression of TaRaf46 decreased wheat resistance to Pst and repressed MAPK activation in wheat. Knocking down TaRaf46 expression in wheat attenuated Pst infection and increased wheat immunity.
Pst27791 interacting with wheat rapidly accelerated fibrosarcoma (Raf)-like kinase TaRaf46 in yeast and in planta. Transgenic wheat expressing the RNA interference construct of Pst27791 exhibited high resistance to Pst virulent isolate CYR31, indicating its importance in pathogenesis. Stable overexpression of Pst27791 in wheat suppressed reactive oxygen species accumulation and the salicylic acid-dependent defense response. In this study, we identified a serine-rich effector, Pst27791, from Pst that suppresses cell death in Nicotiana benthamiana. Identifying host targets of effector proteins and clarifying their roles in pathogen infection is essential for understanding pathogen virulence. tritici ( Pst) is an important obligate pathogen in wheat ( Triticum aestivum L.) and secretes effectors into plant cells to promote infection.
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